rna extraction from animal and human's cancerous tissues: does tissue matter?

Authors

ali akbar samadani cellular and molecular biology research center, babol university of medical sciences, babol, iran.سازمان اصلی تایید شده: دانشگاه علوم پزشکی بابل (babol university of medical sciences)

novin nikbakhsh department of surgery, babol university of medical sciences, babol, iran.سازمان اصلی تایید شده: دانشگاه علوم پزشکی بابل (babol university of medical sciences)

sadegh fatahi cellular and molecular biology research center, babol university of medical sciences, babol, iran.سازمان اصلی تایید شده: دانشگاه علوم پزشکی بابل (babol university of medical sciences)

seyed mohsen aghajanpour mir cellular and molecular biology research center, babol university of medical sciences, babol, iran.سازمان اصلی تایید شده: دانشگاه علوم پزشکی بابل (babol university of medical sciences)

abstract

the reliability of gene expression profiling based technologies and methods to find transcriptional differences representative for the original samples is influenced by the quality of the extracted rna. hence, rna extraction is the first step to investigate the gene expression and its function. consequently, the quality of extracted rna is really significant. correspondingly, this research was accomplished in order to optimize the rna extraction methods and compare the tissue amounts or tissues quality. relatively, the cancerous tissues of human's stomach in fresh and frozen conditions and also the mouse's fresh tissue were studied. some factors like the amount of samples, efficacy differences of diverse extraction's buffers (tripure, trizol) and also the efficacy of b-mercaptoethanol were compared and investigated. the results indicated that the less amount (1-2 mg) compared to other amounts (2-5 mg, 5-15 mg) yielded the best quality and the rna's bands (5s, 18s, 28s) were observed perfectly. relatively, comparing and measuring some kinds of buffers (trizol, tripure) indicated no difference in rna extraction quality. the last investigated factor was the effect of b- mercaptoethanol which was used along with tripure in order to remove the rnase. conclusively, no effective impression was observed.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

RNA Extraction from Animal and Human\'s Cancerous Tissues: Does Tissue Matter?

The reliability of gene expression profiling based technologies and methods to find transcriptional differences representative for the original samples is influenced by the quality of the extracted RNA. Hence, RNA extraction is the first step to investigate the gene expression and its function. Consequently, the quality of extracted RNA is really significant. Correspondingly, this research was ...

full text

RNA Extraction from Animal and Human's Cancerous Tissues: Does Tissue Matter?

The reliability of gene expression profiling, based technologies and methods to find transcriptional differences representative of the original samples is influenced by the quality of the extracted RNA. Hence, RNA extraction is the first step to investigate the gene expression and its function. Consequently, the quality of extracted RNA is really significant. Correspondingly, this research was ...

full text

Optimization of RNA Extraction from Rat Pancreatic Tissue

Background: Optimized RNA extraction from tissues and cell lines consists of four main stages regardless of the method of extraction: 1) homogenizing, 2) effective denaturation of proteins from RNA, 3) inactivation of ribonuclease, and 4) removal of any DNA, protein, and carbohydrate contamination. Isolation of undamaged intact RNA is challenging when the related tissue contains high levels of ...

full text

optimization of rna extraction from rat pancreatic tissue

background: optimized rna extraction from tissues and cell lines consists of four main stages regardless of the method of extraction: 1) homogenizing, 2) effective denaturation of proteins from rna, 3) inactivation of ribonuclease, and 4) removal of any dna, protein, and carbohydrate contamination. isolation of undamaged intact rna is challenging when the related tissue contains high levels of ...

full text

Cytoplasmic RNA extraction from fresh and frozen mammalian tissues.

The quality of collections of expressed sequence tags andfull-length cDNAs is adversely affected by the presence of "junk" clones derivedfrom unspliced or partially spliced RNAs present in conventional total RNA preparations. One can overcome this problem by using intact cytoplasmic RNA to create cDNA libraries, but the methods in the literature that describe the preparation of RNA only work we...

full text

Does Marriage Matter to Women and Men?

Purpose: the transformation of marriage patterns and family formation by following transition from traditional society to a modern society causes changes in goals, function of family formation. The aim of this study was comparative investigation of the attitudes of single and married individuals about marriage function. Materials and method: the study performed by qualitative method. The statis...

full text

My Resources

Save resource for easier access later


Journal title:
international journal of molecular and cellular medicine

جلد ۴، شماره ۱، صفحات ۵۴-۵۹

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023